Well, it's taken me quite a while to return to this topic, but from the "Fourth Annual Steam Passenger Service Directory-1969" published by the Empire State Railway Museum, here's a list of steam railways and trolley lines that, to the best of my knowledge, no longer operate. I'm sure others have come and gone in the interim but I'll start this off:
Arizona-White Mountain Scenic, used two 2-8-2's on an old logging railroad
Arkansas-Reader Railroad, very well known, used to run mixed trains
California West Side & Cherry Valley Railway 3 foot gauge logging railroad
Colorado Central Narrow Gauge Railway Central City used an outside frame 2-8-0 from the International Railways of Central America
Delaware Red Clay Valley Railway Equipment & Leasing-ran excursions in the Wilmingtom-Baltimore area using two ex-Canadian Pacific 4-6-2's.
Indiana Railway Museum Westport used an 0-4-0T pulling a former trolley car
Michigan-Keweenaw Central Railway, Cadillac and Lake City Railway
New Jersey-Trolley Valhalla, Morris County Central
New York-Leather Stocking Line, 3 foot gauge in Cooperstown, Livionia, Avon and Lakeville (previously mentioned by Otto), Delaware Otsego
North Carolina-Southern Appalachian Railway (used an ex-BEDT 0-6-0T),
Bear Creek Scenic (used 3-truck Shays on the Graham
County RR)
Oregon-Veronia, South Park & Sunset used a 2-6-2 on a SP&S branchline.
Pennsylvania-Penn View Mountain, Carroll Park & Western (4 foot gauge), Everett Railroad, Valley Forge Scenic, Quakertown & Eastern (used ex-Buffalo Creek & Gauley 2-8-0 #4 on excursions in the Quakertown area).
Vermont-Green Mountain Railroad (the original Steamtown)
Virginia-Dry Gulch & Tombstone (used a 3-truck Shay)
Well, that's it for starters, not mentioned in my list were the Southern Railroad (still running steam excursions at that time), the High Iron Company (ex-NKP 759), the Midwest Railway Historical Foundation (used ex-GTW 4070 on excursions in Ohio and western Pa) and the Clinchfield, that used a 4-6-0 in excursion service.
Additions or corrections are welcome.
Art Audley, AA3RT
Moderator: Railroad Radio & Communications, Railroads in Books, Magazines, Music, TV and other Media, General Discussion: Fallen Trolley & Interurban Lines, General Discussion: Shortline, Industrial & Military Railroads,