Speaking from personal experience, it is not always easy to get into the railroad even with higher education and people on the inside putting in a good word for you. I work for the largest rail carrier in the US and with a higher education and good grades and a good work history and knowing several people on the inside it still took me several applications over a period of a couple of years to get my foot in the door. Everyone wants to work for the railroad and there is a lot of competition for the jobs. Also, don't expect to get your first job at the railroad and automatically be a conductor right away just because you took some classes. You need to take any job you can possibly get working for the railroad. Get your foot in the door doing anything.
With all that being said, if you work hard enough and want it bad enough you will succeed. Don't give up and don't get discouraged. If you are a safe, hard worker and bang on the door long enough, eventually someone will let you in. Good luck to you.
P.S. And if you do get in, you may hear a lot of griping and negativity. Don't listen to any of it. Your job will be what you make of it.