New York State Railways (trolley) ran down the middle of Lyell from Lake Ave to Glide St. At Glide there was a turn around loop. Don't know when this was removed ? Post WWII ? I lived near Lyell and Glide starting in 1982, there were no tracks left in the middle of Lyell Ave at that time.
The RL&B was just north of the Falls Road, Trolley Blvd in Gates was the RL&B roadbed. East of the end of Trolley Blvd at the Barge Canal are two (pretty impressive) railroad bridges over the Canal. The southern one (double tracked) was the NYCRR Falls Road, the northern one was the RL&B. There were still rails crossing Lee Rd (the NYCRR) until about 10 years ago (maybe 15).
Not aware of any "Class One" railroad tracks running down Lyell. The Falls Road (NYCRR) and the BR&P (B&O) still cross Lyell at grade just east and west of Mt. Read Blvd. The Charlotte Branch crosses Lyell at grade further east of Mt. Read. The Ragu plant was about a mile west of the BR&P crossing, the Falls Road was north of the Ragu plant, I don't think they had a siding.
Lots of good info at this web site, there were RR and Trolley lines all over the place.
https://maps.cityofrochester.gov/historic/" onclick="window.open(this.href);return false;
Edit, I checked the 1910 map at that website, the RL&B did run down the middle of Lyell Ave starting at Glide St. (then called Kossuch St.) and heading east towards Lake Ave. The 1935 map does not show the RL&B any longer. It shows a turnaround loop at the Northwest corner of Glide and Lyell. Maybe the RL&B had "trackage rights" into the City itself ?
Cheers, Kevin.